Motility (CASA) | Breed Diagnostics

Motility Assessment (CASA)

Motility is objectively assessed using computer assisted sperm assessment (CASA). An aliquot of processed spermatozoa is loaded into the CASA slides (containing cell chambers) and maintained at 35 °C to prevent any loss of motility through cold shock. Sperm motility is tracked using real-time video capture and assessed using the CASA software. The following parameters are reported:

Total motility: Percentage of motile spermatozoa
Progressive motility: Percentage of motile spermatozoa moving in a forward, progressive manner
Straight line velocity (VSL): Time-average velocity along the straight line between the spermatozoon's first and last detected points
Curvilinear velocity (VCL): Average velocity measured over the actual point-to-point track followed by the cell.
Average path velocity (VAP): Measures the sperm head along its spatial average trajectory
Linearity: Linearity of the curvilinear trajectory (VSL/VCL × 100)
Straightness: The linearity of the sperm average path (VSL/VAP × 100)

Several studies have shown that CASA parameters can accurately predict male fertility status [1-5]. The fundamentals of CASA and its applications in livestock fertility are discussed in detail by Amann and Waberski [6] and Mortimer [7].

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References

1. Aitken, R.J., Best, F.S., Warner, P., and Templeton, A. (1984) A prospective study of the relationship between semen quality and fertility in cases of unexplained infertility. Journal of andrology. 5(4): p. 297-303.

2. Krause, W. (1995) Computer-assisted semen analysis systems: comparison with routine evaluation and prognostic value in male fertility and assisted reproduction. Human Reproduction. 10(suppl_1): p. 60-66.

3. Larsen, L., Scheike, T., Jensen, T.K., Bonde, J.P., Ernst, E., Hjollund, N.H., Zhou, Y., Skakkebæk, N.E., Giwercman, A., et al. (2000) Computer-assisted semen analysis parameters as predictors for fertility of men from the general population. Human Reproduction. 15(7): p. 1562-1567.

4. Farrell, P.B., Presicce, G.A., Brockett, C.C., and Foote, R.H. (1998) Quantification of bull sperm characteristics measured by computer-assisted sperm analysis (CASA) and the relationship to fertility. Theriogenology. 49(4): p. 871-9.

5. Love, C.C. (2011) Relationship between sperm motility, morphology and the fertility of stallions. Theriogenology. 76(3): p. 547-557.

6. Amann, R.P. and Waberski, D. (2014) Computer-assisted sperm analysis (CASA): capabilities and potential developments. Theriogenology. 81(1): p. 5-17. e3.

7. Mortimer, S.T. (2000) CASA-practical aspects. J Androl. 21(4): p. 515-24

Semen Assessments

Motility Assessment (CASA)

References